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Addgene inc aav2 retro

<t>AAV2-retro</t> <t>preferentially</t> transduces photoreceptors and RPE 1 and 3 days after intravitreal injection. ( A , C ) Stitched 10× images of retinal cross-sections showing mGL expression at 1 dpi ( A ) and at 3 dpi ( C ). Blue indicates DAPI-labeled nuclei; green indicates mGL reporter expression. ( B , D ) Higher-magnification images of representative retinal regions showing mGL expression at 1 dpi ( B ) and at 3 dpi ( D ). ( B’ , D’ ) Merged images corresponding to panels B and D , illustrating mGL and DAPI expression across retinal layers. Retinal layers are indicated: RPE, ONL, OPL, INL, inner plexiform layer (IPL), and GCL. Scale bars : 200 µm ( A , C ); 50 µm ( B’ , D’ ).

Aav2 Retro, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/raav2/rAAV2-retro+helper+(Plasmid+%2381070)/pmc13037736-28-7-19
Average 95 stars, based on 90 article reviews

aav2 retro - by Bioz Stars, 2026-09

95/100 stars

Images

1) Product Images from "AAV2-Retro–Mediated Gene Transfer Selectively Targets Outer Retinal Cells Following Intravitreal Injection"

Article Title: AAV2-Retro–Mediated Gene Transfer Selectively Targets Outer Retinal Cells Following Intravitreal Injection

Journal: Investigative Ophthalmology & Visual Science

doi: 10.1167/iovs.67.3.54

AAV2-retro preferentially transduces photoreceptors and RPE 1 and 3 days after intravitreal injection. ( A , C ) Stitched 10× images of retinal cross-sections showing mGL expression at 1 dpi ( A ) and at 3 dpi ( C ). Blue indicates DAPI-labeled nuclei; green indicates mGL reporter expression. ( B , D ) Higher-magnification images of representative retinal regions showing mGL expression at 1 dpi ( B ) and at 3 dpi ( D ). ( B’ , D’ ) Merged images corresponding to panels B and D , illustrating mGL and DAPI expression across retinal layers. Retinal layers are indicated: RPE, ONL, OPL, INL, inner plexiform layer (IPL), and GCL. Scale bars : 200 µm ( A , C ); 50 µm ( B’ , D’ ).

Figure Legend Snippet: AAV2-retro preferentially transduces photoreceptors and RPE 1 and 3 days after intravitreal injection. ( A , C ) Stitched 10× images of retinal cross-sections showing mGL expression at 1 dpi ( A ) and at 3 dpi ( C ). Blue indicates DAPI-labeled nuclei; green indicates mGL reporter expression. ( B , D ) Higher-magnification images of representative retinal regions showing mGL expression at 1 dpi ( B ) and at 3 dpi ( D ). ( B’ , D’ ) Merged images corresponding to panels B and D , illustrating mGL and DAPI expression across retinal layers. Retinal layers are indicated: RPE, ONL, OPL, INL, inner plexiform layer (IPL), and GCL. Scale bars : 200 µm ( A , C ); 50 µm ( B’ , D’ ).

Techniques Used: Injection, Expressing, Labeling

AAV2-retro maintains selective transduction in the outer layers 14 days after intravitreal injection. ( A ) Stitched 10× images of retinal cross-sections showing mGL expression at 14 dpi. ( B ) Higher-magnification view of a representative retinal region with mGL expression. ( B’ ) Merged image from B highlighting layer-specific distribution (RPE, ONL, OPL, INL, IPL, and GCL), mGL, and DAPI across the layers. ( C ) Retinal flatmount at 14 dpi after sequential injections, showing widespread mGL expression. ( C’ , C’’ ) Higher magnification images of the boxed areas in C . Scale bars : 200 µm ( A , C ); 50 µm ( B’ , C’ , C’’ ). ( D ) Quantification of mGL-positive cells within the regions of high transduction in the ONL (i.e., ROI). The percentage shown indicates the proportion of transduced cells among total DAPI-positive ONL cells. N = 3 animals. Scale bars : 200 µm ( A , C ); 50 µm ( C’ , C’’ ).

Figure Legend Snippet: AAV2-retro maintains selective transduction in the outer layers 14 days after intravitreal injection. ( A ) Stitched 10× images of retinal cross-sections showing mGL expression at 14 dpi. ( B ) Higher-magnification view of a representative retinal region with mGL expression. ( B’ ) Merged image from B highlighting layer-specific distribution (RPE, ONL, OPL, INL, IPL, and GCL), mGL, and DAPI across the layers. ( C ) Retinal flatmount at 14 dpi after sequential injections, showing widespread mGL expression. ( C’ , C’’ ) Higher magnification images of the boxed areas in C . Scale bars : 200 µm ( A , C ); 50 µm ( B’ , C’ , C’’ ). ( D ) Quantification of mGL-positive cells within the regions of high transduction in the ONL (i.e., ROI). The percentage shown indicates the proportion of transduced cells among total DAPI-positive ONL cells. N = 3 animals. Scale bars : 200 µm ( A , C ); 50 µm ( C’ , C’’ ).

Techniques Used: Transduction, Injection, Expressing

AAV2-retro leads to similar preferential transduction in female mice after intravitreal injection. ( A , C ) Stitched 10× images of retinal cross-sections showing mGL expression at 3 dpi ( A ) and at 14 dpi ( C ). Blue indicates DAPI-labeled nuclei; green indicates mGL reporter expression. ( B , D ) Higher magnification images of representative retinal regions showing mGL expression at 3 dpi ( B ) and at 14 dpi ( D ). ( B’ , D’ ) Merged images corresponding to panels B and D , illustrating mGL and DAPI expression across retinal layers. Retinal layers are indicated: RPE, ONL, OPL, INL, IPL, and GCL. Three female mice were examined per time point with similar results. Scale bars : 200 µm ( A , C ); 50 µm ( B’ , D’ ).

Figure Legend Snippet: AAV2-retro leads to similar preferential transduction in female mice after intravitreal injection. ( A , C ) Stitched 10× images of retinal cross-sections showing mGL expression at 3 dpi ( A ) and at 14 dpi ( C ). Blue indicates DAPI-labeled nuclei; green indicates mGL reporter expression. ( B , D ) Higher magnification images of representative retinal regions showing mGL expression at 3 dpi ( B ) and at 14 dpi ( D ). ( B’ , D’ ) Merged images corresponding to panels B and D , illustrating mGL and DAPI expression across retinal layers. Retinal layers are indicated: RPE, ONL, OPL, INL, IPL, and GCL. Three female mice were examined per time point with similar results. Scale bars : 200 µm ( A , C ); 50 µm ( B’ , D’ ).

Techniques Used: Transduction, Injection, Expressing, Labeling

AAV2-retro effectively delivers transgene to both rods and cones. ( A , B ) Representative retinal cross-sections showing mGL expression ( green ) at 3 dpi ( A ) and at 14 dpi ( B ) following intravitreal injection of AAV2-retro–CMV–mGL. Cone arrestin ( magenta ) was used to identify cone photoreceptors. Bottom panel in A shows higher magnification images highlighting the colocalization of mGL with cone arrestin immunoreactivity. Scale bars : 50 µm ( A , B ); 5 µm (higher magnification images).

Figure Legend Snippet: AAV2-retro effectively delivers transgene to both rods and cones. ( A , B ) Representative retinal cross-sections showing mGL expression ( green ) at 3 dpi ( A ) and at 14 dpi ( B ) following intravitreal injection of AAV2-retro–CMV–mGL. Cone arrestin ( magenta ) was used to identify cone photoreceptors. Bottom panel in A shows higher magnification images highlighting the colocalization of mGL with cone arrestin immunoreactivity. Scale bars : 50 µm ( A , B ); 5 µm (higher magnification images).

Techniques Used: Expressing, Injection

AAV2-retro transduces only a small number of amacrine cells and RGCs. ( A , B ) Retinal cross-sections show mGL expression ( green ) at 3 dpi ( A ) and at 14 dpi ( B ). AP-2α immunoreactivity is shown in magenta and RBPMS immunoreactivity in red . (A’ , B’ ) Higher magnification images showing cells that appear to be co-labeled with mGL and AP-2α. ( C ) Quantification shows that the percentage of mGL + cells that were positive for either RBPMS (in GCL and INL) or AP-2α at each time point. N = 3 per group. Error bar, ± SEM Two-way ANOVA with Tukey's multiple comparisons: * < 0.05, **** < 0.0001, ns = not significant. Scale bars : 50 µm ( A , B ); 5 µm ( A’ , B’ ).

Figure Legend Snippet: AAV2-retro transduces only a small number of amacrine cells and RGCs. ( A , B ) Retinal cross-sections show mGL expression ( green ) at 3 dpi ( A ) and at 14 dpi ( B ). AP-2α immunoreactivity is shown in magenta and RBPMS immunoreactivity in red . (A’ , B’ ) Higher magnification images showing cells that appear to be co-labeled with mGL and AP-2α. ( C ) Quantification shows that the percentage of mGL + cells that were positive for either RBPMS (in GCL and INL) or AP-2α at each time point. N = 3 per group. Error bar, ± SEM Two-way ANOVA with Tukey's multiple comparisons: * < 0.05, **** < 0.0001, ns = not significant. Scale bars : 50 µm ( A , B ); 5 µm ( A’ , B’ ).

Techniques Used: Expressing, Labeling

Injection:

Article Title: Hypothalamic control of interoceptive hunger.
Article Snippet: For all experiments, 50 nL of an adeno-associated virus cocktail was injected bilaterally (rate: 30 nL/min) into the ARC (bregma: 1.70mm;midline: ± 0.25mm; dorsal skull surface: 5.85, 5.80 and 5.75 mm; AgrpCre mice) or LH (bregma: 1.23 mm; midline: ± 1.00 mm; dorsal skull surface: 5.15 mm; VgatCre and Vglut2Cre mice) by a pulled glass pipette (20 – 30 mm tip diameter) with a micromanipulator (Narishige International USA, NY, USA) controlling the injection speed. .. One of two viral cocktails was injected: (1) Experimental: rAAV2/9-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (Addgene viral prep 20298-AAV9) + rAAV2/9-hSyn-DIO-hM4D(Gi)-mCherry (Addgene viral prep 44362-AAV9), or (2) Control: rAAV2/9-EF1a-DIO-EYFP (Addgene viral prep 27056-AAV9) + rAAV2/9-hSyn-DIO-mCherry (Addgene viral prep 50459-AAV9). ..

Control:

In Vivo:

Article Title: Anterior hypothalamic parvalbumin neurons are glutamatergic and promote escape behavior.
Article Snippet: .. For in vivo functional imaging experiments, a GRIN lens (500 mm, Snap-in imaging cannula Model L-V; Doric Lenses, Inc., Qu ebec, QC, Canada) was implanted to record AHA neuronal activity (AP: 0.85, ML: +0.425, DV: 5.3) as described previously.73 Viruses used include: 1. rAAV2/9-CAG-FLEX-jGCaMP8f-WPRE, titer: 5.03 1012 GC/ml (Addgene viral prep # 162382-AAV9, RRID:Addgene_162382; Addgene, MA, U.S.A.)15, 2. rAAV2/9-SYN-FLEX-jGCaMP7s-WPRE, titer: 5.03 1012 GC/ml (Addgene viral prep # 104491-AAV9, RRID:Addgene_104491; Addgene, MA, U.S.A.)16, 3. rAAV2/9-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA, titer: 5.0 3 1012 GC/ml (Addgene viral prep # 20298-AAV9, RRID:Addgene_20298)74, 4. rAAV2/9-EF1a-DIO-EYFP, titer: 5.0 3 1012 GC/ml (Addgene viral prep # 27056-AAV9, RRID:Addgene_27056)74, 5. rAAV2/8-EF1a-DIO-iC++-EYFP, titer: 5.03 1012 GC/ml (Cat #GVVC-AAV-108; Stanford NeuroscienceGene Vector and Virus Core, CA, U.S.A.), 6. rAAV2/1-EF1a-FLEX-taCasp3-TEVp, titer: 1.93 1012 GC/ml (RRID:Addgene_45580; University of North Carolina Vector Core NC, U.S.A.)37, 7. rAAV2/1-CAG-FLEX-EGFP-WPRE, titer: 5.0 3 1012 GC/ml (Cat # AV-1-ALL854, RRID:Addgene_51502; University of Pennsylvania (U Penn) Vector Core, PA, U.S.A.)75, 8. rAAV2/1-EF1a-DIO-synaptophysin (SYP)-mCherry, titer: 5.03 1012 GC/ml (Cat # AAV-RN1; Massachusetts General Hospital Gene Delivery Technology Core, MA, U.S.A.)33, 9. rAAV2/1-CAG-FLEX-ChR2-tdTomato, titer: 53 1012 GC/ml (Addgene viral prep # 18917-AAV1, RRID:Addgene_18917),76,78 and 10. .. AAV2/1-CAG-FLEX-tdTomato, titer: 4.5 3 1013 GC/ml (Cat # AV-1-ALL864, RRID:Addgene_51503; U Penn Vector Core).75 In vivo functional imaging and predatory threat exposure For in vivo functional imaging the Cre-dependent genetically encoded calcium indicators jGCaMP8f and jGCaMP7s were used for visualization of neuronal activity in Slc32a1Cre and PvalbCre mice.

Functional Assay:

Imaging:

Activity Assay:

Virus:

Article Title: Anterior hypothalamic parvalbumin neurons are glutamatergic and promote escape behavior
Article Snippet: For in vivo functional imaging experiments, a GRIN lens (500 μm, Snap-in imaging cannula Model L-V; Doric Lenses, Inc., Québec, QC, Canada) was implanted to record AHA neuronal activity (AP: −0.85, ML: +0.425, DV: −5.3) as described previously ( ). .. Viruses used include: (1) rAAV2/9-CAG-FLEX-jGCaMP8f-WPRE, titer: 5.0 × 10 12 GC/ml (Addgene viral prep # 162382-AAV9, RRID:Addgene_162382; Addgene, MA, U.S.A.), (2) rAAV2/9-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA, titer: 5.0 × 10 12 GC/ml (Addgene viral prep # 20298-AAV9, RRID:Addgene_20298), (3) rAAV2/9-EF1a-DIO-YFP, titer: 5.0 × 10 12 GC/ml (Addgene viral prep # 27056-AAV9, RRID:Addgene_27056), (4) rAAV2/8-EF1a-DIO-iC++-EYFP, titer: 5.0 × 10 12 GC/ml (Cat # GVVC-AAV-108; Stanford Neuroscience Gene Vector and Virus Core, CA, U.S.A.), (5) rAAV2/1-EF1a-FLEX-taCasp3-TEVp, titer: 1.9 × 10 12 GC/ml (RRID:Addgene_45580; University of North Carolina Vector Core NC, U.S.A.), (6) rAAV2/1-CAG-FLEX-EGFP-WPRE, titer: 5.0 × 10 12 GC/ml (Cat # AV-1-ALL854, RRID:Addgene_51502; University of Pennsylvania (U Penn) Vector Core, PA, U.S.A.), (7) rAAV2/1-EF1a-DIO-synaptophysin (SYP)-mCherry, titer: 5.0 × 10 12 GC/ml (Cat # AAV-RN1; Massachusetts General Hospital Gene Delivery Technology Core, MA, U.S.A.), (8) rAAV2/1-CAG-FLEX-ChR2-tdTomato, titer: 5 × 10 12 GC/ml (Addgene viral prep # 18917-AAV1, RRID:Addgene_18917), and (9) rAAV2/1-CAG-FLEX-tdTomato, titer: 4.5 × 10 13 GC/ml (Cat # AV-1-ALL864, RRID:Addgene_51503; U Penn Vector Core). ..

Bioprocessing:

Plasmid Preparation:

Article Title: A comparison of viral strategies and model systems to target norepinephrine neurons in the locus coeruleus reveals high variability in transgene expression patterns.
Article Snippet: .. Injections of rAAV2/9-Ef1a-DO-DIO-tdTomato-eGFP (2.3 × 1013 gc/ml; AddGene plasmid #37120) [45] into the LC were performed in Dbhcre, Netcre, and Thcre animals. ..

Article Title: A comparison of viral strategies and model systems to target norepinephrine neurons in the locus coeruleus reveals high variability in transgene expression patterns.
Article Snippet: .. We thank Prof. Dr. Bernardo Sabatini for kindly gifting the rAAV2/9-EF1a-DO-DIOtdTomato-eGFP construct (AddGene plasmid #37120), the GENIE Project for gifting pGP-AAV-syn-jGCaMP8m-WPRE (AddGene plasmid #162375), Douglas Kim, PhD, and the GENIE Project for gifting pGP-CMV-NES-jRGECO1a (AddGene plasmid #61563), and Prof. Dr. Susumu Tonegawa for kindly providing the Netcre line. ..

Construct:

Article Title: A comparison of viral strategies and model systems to target norepinephrine neurons in the locus coeruleus reveals high variability in transgene expression patterns.
Article Snippet: .. We thank Prof. Dr. Bernardo Sabatini for kindly gifting the rAAV2/9-EF1a-DO-DIOtdTomato-eGFP construct (AddGene plasmid #37120), the GENIE Project for gifting pGP-AAV-syn-jGCaMP8m-WPRE (AddGene plasmid #162375), Douglas Kim, PhD, and the GENIE Project for gifting pGP-CMV-NES-jRGECO1a (AddGene plasmid #61563), and Prof. Dr. Susumu Tonegawa for kindly providing the Netcre line. ..

other:

Article Title: Activation of the fear-responsive anterior hypothalamic area promotes avoidance and triggers compulsive grooming behavior in mice
Article Snippet: Viruses used include: (1) rAAV2/1-Camk2a-hChR2(H134R)-EYFP-WPRE, titer: 5.0 × 10 GC/ml (Addgene viral prep # 26969-AAV1, RRID:Addgene_26969; Addgene, MA, U.S.A.) , (2) rAAV2/1-Camk2a-eYFP-WPRE, titer: 5.0 × 10 GC/ml (Addgene viral prep # 105622-AAV1, RRID:Addgene_105622), (3) rAAV2/9-Syn-jGCaMP7s-WPRE, titer: 5.0 × 10 GC/ml (Addgene viral prep # 104487-AAV1, RRID:Addgene_27056) .

Review